Host: Mouse 

Clonality: Monoclonal 

Isotype: IgG1 

CloneNo.: 9F11 

Applications: 

IHC-P (1:200-1000) 

IHC-F (1:200-1000)

IF (1:200-1000) 

Flow-Cyt (1ug/Test) 

ICC/IF (1:100-500) 

ELISA (1:1000-5000) 

IP (1:50-200) 

Reactivity: Species independent O

bserved 70 kDa 

Subcellular Location: Cytoplasm ,Nucleus 

Target: mCherry 

Purification: affinity purified by Protein G 

Concentration: 1mg/ml 

Storage: Size : 100ul/500ul 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Size : 200ug (PBS only) 0.01M PBS Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. 

Background: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.

mCherry tag was immunoprecipitated from mCherry expression vector whole cell lysate with at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP conjugated Goat Anti- Mouse IgG (H&L) was used as secondary antibody. Lane 1: mCherry expression vector whole cell lysate (Input). Lane 2: IP in mCherry expression vector whole cell lysate. Lane 3: native mouse IgG IP in mCherry expression vector whole cell lysate (negative control).