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IRF3 Rabbit pAb
BES25606pAb
Host: Rabbit Clonality: Polyclonal GeneID: 3661 Target: IRF3 | Isotype: IgG
SWISS: Q14653 | Applications: WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (2ug/Test) Reactivity: Human, Mouse, Rat (predicted: Pig, Cow, Dog, Horse)
Predicted
Observed
Subcellular Location: Cytoplasm ,Nucleus |
Immunogen: KLH conjugated synthetic peptide derived from human IRF3: 51-150/427. Puri?cation: affinity purified by Protein A Concentration: 1mg/ml Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Background: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]. | ||
47 kDa
50 kDa 
Sample: Heart(Mouse) Lysate at 40 ug Heart(Rat) Lysate at 40 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-IRF3 at 1/1000 dilution
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD
Observed band size: 50 kD
Tissue/cell: human kidney tissue; 4%
Paraformaldehyde-fixed and paraffin-
embedded; Antigen retrieval: citrate buffer (
0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen
peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation:
Anti-IRF3 Polyclonal Antibody,
Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary
antibody and DAB staining
Tissue/cell: human colon carcinoma; 4%
Paraformaldehyde-fixed and paraffin-
embedded; Antigen retrieval: citrate buffer (
0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen
peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation:
Anti-IRF3 Polyclonal Antibody,
Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary
Antibody and DAB staining
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