血管內皮生長因子D(VEGFD)活性蛋白

Active Vascular Endothelial Growth Factor D (VEGFD)

FIGF; VEGF-D; C-Fos Induced Growth Factor

貨號：BES4673AP

規格：10ug/50μg/200μg/1mg

物種：Homo sapiens (Human，人) 相同的名稱，不同的物種。

緩沖液成份：20mM Tris, 150mM NaCl

緩沖液：（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

性狀：凍干粉

純度：> 90%

等電點：-

應用：Cell culture; Activity Assays.

活性實驗：

Vascular Endothelial Growth Factor D (VEGFD) is a crucial member of the VEGF family, encoded by the VEGFD gene in humans. It is synthesized as an inactive precursor protein and activated via proteolytic cleavage. VEGFD exerts biological functions primarily through binding to two specific receptors, VEGFR-2 and VEGFR-3, expressed on vascular and lymphatic endothelial cells. These bindings trigger downstream signaling pathways, regulating angiogenesis (new blood vessel formation) and lymphangiogenesis (lymphatic vessel development). Beyond physiological processes like embryonic development and tissue repair, abnormal VEGFD expression is closely linked to pathological conditions, especially cancer. It promotes tumor angiogenesis and lymphatic metastasis, making it a potential therapeutic target for cancer and other angiogenesis-related disorders.To detect the activity of recombinant VEGFD , a functional ELISA assay was performed to evaluate the interaction between recombinant human VEGFD and recombinant rat FGFR1.Briefly, VEGFD was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VEGFD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human VEGFD and recombinant rat FGFR1 was shown in Figure 1, the EC50 for this effect is 0.12881μg/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。

特別提示：本公司的所有產品僅可用于科研實驗，嚴禁用于臨床醫療及其他非科研用途！以實際收貨產品說明書為準，網站說明書僅供參考。